Asian Frozen Eggs Fertilization Guaranteed Program-亚洲卵子库

Asian Frozen Eggs Fertilization Guaranteed Program Policy

1. This policy is applicable to clinics/laboratories that are accredited by Asian Frozen Eggs.
2. This policy is applicable when patient(s) purchases no less than 8 mature frozen oocytes (MII) at a time.
3. This policy is applicable when the quality of the sperm sample(s) is no worse than Reference Value for Human Semen Characteristics by World Health Organization, 5th edition, 2010,Appendix I.
4. This policy is applicable when DNA Fragmentation Rate of the sperm sample(s) is less than 20%.
5. This policy is applicable to patient(s) with a fertilization rate less than 67% out of all purchased MIIs.
6. This policy is applicable when all embryos created from the purchase has been successfully transferred and no clinical pregnancy has achieved. (B-Hcg Test result lesser than 30 IU/L, 14 days post transfer)

1. The embryology laboratory that manipulates frozen gametes should follow the Standard Operation Protocol provided by Asian Frozen Eggs strictly in MII thawing procedure.
2. The embryology laboratory that manipulates frozen gametes should only use ICSI as fertilization method.
3. Normal fertilization refers to 2 pronuclei fertilization 16-20 hours after the sperm being injected into recovered oocytes.
4. The guideline recovery rate is 90% out of all mature frozen MII.
5. The guideline fertilization rate is 75% out of recovered MII.
6. The guideline fertilization rate is 67% out of all mature frozen MII.

1. Each purchase is only eligible for one replacement.
2. In failed/partially failed fertilization cases, Asian Frozen Eggs will replace a number of MIIs in accordance to the fertilization result.
2.1 When the fertilization rate is between 50%-67%, Asian Frozen Eggs shall replace 20% of the total number of MIIs purchased.
2.2 When the fertilization rate is between 25%-50%, Asian Frozen Eggs shall replace 40% of the total number of MIIs purchased.
2.3 When the fertilization rate is lower than 25%, Asian Frozen Eggs shall replace 100% of the total number of MIIs purchased.
2.4 Appendix II demonstrate individual cases explicitly.
3. Asian Frozen Eggs does not guarantee that the replacement MIIs are from the same egg donor as their initial purchase.

Parameter	Lower Reference Limit
Semen Volume (ml)	1.5 (1.4-1.7)
Total Sperm Number (106 per ejaculate)	39 (33-46)
Sperm Concentration (106 per ml)	15 (12-16)
Total Motility (PR+NP, %)	40 (38-42)
Progressive Motility (PR, %)	32 (31-34)
Vitality (Live Spermatozoa, %)	58 (55-63)
Sperm Morphology (Normal Forms, %)	4 (3.0-4.0)
Other Consensus Threshold Values
pH	>=7.2
Peroxidase-positive Leukocytes (106 per ml)	<1.0
MAR test (Motile Spermatozoa With Bound Particles, %)	<50
Immunobead Test (Motile Spermatozoa With Bound Beads, %)	<50
Seminal Zinc (umol/ejaculate)	>=2.4
Seminal Fructose (u mol/ejaculate)	>=13
Seminal Neutral Glucosidase (mU/ejaculate)	>=20

Reference Box			0.5~0.67		0.25~0.5		0~0.25
MII No.	Fert Rate	Fert MIIs	Range 1	Rep No.	Range 2	Rep No.	Range 3	Rep No.
8	0.67	5	4	2	2 to 3	3	0 to 1	8
9	0.67	6	5	2	3 to 4	4	0 to 2	9
10	0.67	7	6	2	3 to 5	4	0 to 2	10
11	0.67	7	6	2	3 to 5	5	0 to 2	11
12	0.67	8	6 to 7	2	3 to 5	6	0 to 2	12
13	0.67	9	7 to 8	3	4 to 6	6	0 to 3	13
14	0.67	9	7 to 8	3	4 to 6	6	0 to 3	14
15	0.67	10	8 to 9	3	4 to 7	6	0 to 3	15
16	0.67	11	8 to 9	3	4 to 7	6	0 to 3	16
17	0.67	11	9 to 10	3	5 to 8	6	0 to 4	17
18	0.67	12	9 to 11	4	5 to 8	7	0 to 4	18
19	0.67	13	10 to 12	4	5 to 9	8	0 to 4	19
20	0.67	13	10 to 12	4	5 to 9	8	0 to 4	20